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Serotonin has not only been shown to regulate sleep, but it also is responsible for controlling mood, including feelings of optimism, relaxation, general sense of well-being, and the ability to focus and concentrate. When serotonin levels drop, it can lead to a lowered mood, which is what people experience with seasonal affective disorder, premenstrual syndrome, and general stress. People who experience these conditions also have been shown to experience decreased levels of tryptophan, which is responsible for the decrease in production of serotonin. Tryptophan depletion has been associated with a lowering in mood of normal healthy men. In one study, women who had recovered from major depression and ended drug treatment experienced temporary but clinically significant depressive symptoms after tryptophan depletion. In many studies that were performed in the 1970s, indications of trytophan’s ability to relieve lowered mood were found.